Gram-Positive Bacilli: Corynebacterium to Bacillus

Notes

[Diapositiva 1] Gram Positive Bacilli [Diapositiva 2] Gram Positive Bacilli Listeria Erysipelothrix Lactobacillus * Bacillus Corynebacterium Clostridium * Cutibacterium (Propionibacterium *) Mycobacterium Actinomyces * Nocardia [Diapositiva 3] Corynebacterium gpb, cat pos., aerobic or facultative, non-spore formers, nonmotile Morphology: club shaped, L's, V's Chinese characters environment, normal flora of humans, animals and plants major pathogen: C. diphtheriae Others: “diphtheroids” C. jeikeium [Diapositiva 4] Corynebacterium diphtheriae specimen of choice for recovery throat, nasopharynx, wounds [Diapositiva 5] Growth Requirements SBA Loeffler agar Cystine tellurite blood agar (CTB or Tinsdale) 35-37, 24 - 48 hours [Diapositiva 6] Cultural Characteristics morphology is influenced by biotype, media, and stain Loeffler: pleomorphic and beaded with round or pointed ends Stain with methylene blue: metachromatic granules (reddish) at the ends of the rod stored polymerized polyphosphate AKA Babès -Ernst Granule [Diapositiva 7] Biochemical Identification presumptive ID: Patient history, cellular and colonial morphology common biochems are: growth on Tinsdale with halo glucose fermenta...