MLT 140 Fi

Notes

MLT 140 Final Exam Review Fall 2021 What have we learned? Unit 1 History of Microbiology – Leeuwenhoek (1632-1723), Pasteur (1822-1895), Koch (1843-1910), Fleming (1881-1955) Prokaryotic (bacteria) versus Eukaryotic cell (plants, insects, fungi, man) Prokaryotic cell: single cell, binary fission, no nucleus or organelles Eukaryotic cell: single cell or multi-celled, membrane bound nucleus and/or organelles (epithelial cell, white blood cell) Bacteria: capsule, cell wall cell membrane, cytoplasm, DNA in cytoplasm, ribosomes, flagella, pili, endospore ( Bacillus species) Bacteria shapes (cocci, rods, spirochete) Bacteria arrangements: cocci – pairs, chains, diplococci rods – single, chains Bacteria growth requirements: source of carbon and energy, osmotic pressure, moisture, atmosphere, temperature, pH Bacterial growth curve, generation time (Bn=Bo X 2 n) Making media. Use of autoclave. Monitoring autoclave. Storage of media (refrigerator), room temperature before use Mechanical (gauges), Chemical (tape), Biological ( Bacillus spores) Disinfectants: 10% bleach, Vesphene (phenol) Antiseptics: 70% isopropyl alcohol, ethyl alcohol (Purell), tincture of iodine, chlorhexidine, antiseptic ...