CLINICAL B

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CLINICAL BACTERIOLOGY BS MEDICAL LABORATORY SCIENCE ACADEMIC YEAR 2024-2025 (1st Semester) PROFESSOR: [TRANS] ENTEROBACTERIACEAE - TRUE PATHOGENS OUTLINE I Introduction II Shigella spp. A Determinants of Pathogenicity of Shigella spp. III Salmonella spp. A Determinants of Pathogenicity of Salmonella spp. B ARIZONA (Salmonella Enteric Subgroup 3-Arizonae IV Yersinia spp. A Yersinia pestis B Yersinia enterocolitica INTRODUCTION ■ A/A- acid production both in the slant and butt; glucose, sucrose, and lactose fermenter ■ K/K- no acid production; no carbohydrate fermentation ○ LIA (Lysine Iron Agar)- detection of microorganisms ability to decarboxylate lysine, ferment glucose, and we also have your deamination. The following are the reactions: ■ K/K- lysine decarboxylation positive; no glucose fermentation ■ K/A- glucose fermentation ■ R/A- lysine deamination positive; glucose fermentation ● We also have to understand that we have different types of selective media for Enterobacteriaceae and these microorganisms, yung iba sa kanila ay lactose fermenters, yung iba non-lactose fermenters, so a very good example of selective media that we use to identify them based on their lactose ferment...