DTU framework and the need for multi-marker typing in Trypanosoma cruzi

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Am. J. Trop. Med. Hyg., 81(6), 2009, pp. 1041–1049 doi:10.4269/ajtmh.2009.09-0305 Copyright © 2009 by The American Society of Tropical Medicine and Hygiene 1041 * Address correspondence to Michael D. Lewis, Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. E-mail: michael.lewis@lshtm.ac.uk Genotyping of Trypanosoma cruzi: Systematic Selection of Assays Allowing Rapid and Accurate Discrimination of All Known Lineages Michael D. Lewis,* Jonathan Ma, Matthew Yeo, Hernán J. Carrasco, Martin S. Llewellyn, and Michael A. Miles Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom; Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela Abstract. Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic mark- ers are available to determine the DTU to which a T. cruzi...