DNA

Notes

DNA REPLICATION Dr. H. Kavya Naik DNA Replication At the end of this session you will be able to understand the : • Components involved in DNA Replication • Steps of DNA Replication • Difference between Prokaryotic and Eukaryotic DNA replication • Mechanisms of DNA proof reading 2 Recall-DNA is a double helix Two DNA chains spiral around an axis with sugar phosphate backbones on the outside and flat pair of bases meeting in the middle One chain runs in 5’ to 3’upwards and the other runs 5’ to 3’ downwards (Antiparallel) The two chains wrap around each other every ten bases pairs The two chains are held together by complimentary base pairing A with T and G with C (Purine with Pyrimidine) via hydrogen bonding, making it a highly stable molecule 9/4/2023 7 Identify what is wrong with this picture 9/4/2023 9 DNA Replication 12 Semiconservative Replication Semi-Conservative In the semi-conservative model, the two parental strands separate and each makes a copy of itself. After one round of replication, the two daughter molecules each comprises one old and one new strand. Note that after two rounds, two of the DNA molecules consist only of new material, while the other two contain one ol...