Original Article

Apuntes

Original Article Francisco Álvarez-Nava*, Marco Salinas, Daniela Bastidas, Yosselin Vicuña and Marcia Racines-Orbe PPARGC1A promoter DNA-methylation level and glucose metabolism in Ecuadorian women with Turner syndrome https://doi.org/10.1515/hmbci-2020-0076 Received October 22, 2020; accepted November 29, 2020; published online December 22, 2020 Abstract Objectives: Reduced gene expression of PPARGC1A in subjects with insulin resistance (IR) has been reported. Insulin resistance occurs early on the course of Turner syndrome (TS). The main objective of this study was to evaluate the relationship between PPARGC1A promoter DNA methylation status in lymphocytes and insulin sensitivity and secretion in Ecuadorian females with TS. Methods: We examined a cohort of 34 Ecuadorian patients with TS along with a sex-, age- and BMI-matched reference group. All subjects received a standard 75 g oral glucose tolerance test. Insulin resistance and secretion indices were calculated. The PPARGC1A methylated DNA/unmethylated DNA ratio and mitochondrial content (mtDNA/nDNA ratio) were further determined. Results: Notably, the PPARGC1A DNA methylation level was sign...