AGP structure and carbohydrate diversity

Anotacoes

Review Alpha-1-acid glycoprotein Thierry Fournier a , Najet Medjoubi-N b , Dominique Porquet b;* a INSERM U427, Faculte® des Sciences Pharmaceutiques et Biologiques, Universite® Paris 5 Rene® Descartes, Paris, France b Laboratoire de Biochimie Ge®ne®rale, EA 1595, UFR de Pharmacie, 5 rue J.B. Cle®ment, 92296 Chatenay-Malabry, cedex, France Received 19 October 1999; received in revised form 21 January 2000; accepted 8 February 2000 Abstract Alpha-1-acid glycoprotein (AGP) or orosomucoid (ORM) is a 41^43-kDa glycoprotein with a pI of 2.8^3.8. The peptide moiety is a single chain of 183 amino acids (human) or 187 amino acids (rat) with two and one disulfide bridges in humans and rats,respectively. The carbohydrate content represents 45% of the molecular weight attached in the form of five to six highly sialylated complex-type-N-linked glycans. AGP is one of the major acute phase proteins in humans, rats, mice and other species. As most acute phase proteins, its serum concentration increases in response to systemic tissue injury, inflammation or infection, and these changes in serum protein concentrations have been correlated with increases in hepatic synthesis. Expression of the AGP g...